- Post Doctoral
MIT Unit Affiliation:
- Chemical Engineering
- Mechanical Engineering
Post Doc Sponsor / Advisor:
Date PhD Completed:
Top 3 Areas of Expertise:
Expected End Date of Post Doctoral Position:
I have been conducting interdisciplinary research at the frontiers of Acoustics, Micro/Nano Engineering, Drug delivery, Biomedicine, Optics, and Microfluidics. Particularly my research focuses on developing novel microfluidic systems for biomedical applications.
During my postdoctoral research at MIT, I have developed a vector-free microfluidic platform named disruption and field enhancement, in which we break the cell membrane and send material into cells for intracellular delivery. By integrating mechanical disruption and electric field, we are able to deliver versatile range of materials, including proteins, RNAs, DNAs, nano particles/tubes, and quantum dots, into nucleus. In our system, we can directly deliver gene material into nucleus at high throughput, paving a new avenue for intracellular delivery of hard-to-transfer cells such as immune cell. In addition, I demonstrated the co-delivery of DNAs, RNAs, proteins, and dextran in one single step, a critical process for the emerging field of gene editing.
My Ph.D. dissertation at The Pennsylvania State University focuses on a microfluidic platform called Acoustic tweezers. It is able to manipulate nanoparticles, single cells, and whole organisms (such as C. elegans). Our platform has demonstrated 1) Acoustic tweezers based single cell/organism manipulation; 2) Acoustic tweezers based high-efficiency cell separation; 3) Acoustic tweezers based multichannel cell/droplet sorting; 4) Acoustic tweezers based tunable cell patterning. Due to their significance and impact, these research findings have been highlighted at Nature Methods, National Science Foundation (NSF), National Institute of Health (NIH), and reported by more than 300 news and medium outlets, and featured as cover articles at journals Advanced Materials and Lab on a Chip.
Techniques that can noninvasively and dexterously manipulate cells and other bioparticles (such as organisms, DNAs, proteins, and viruses) in a compact system are invaluable for many applications in life sciences and medicine. Historically, optical tweezers have been the primary tool used in the scientific community for bioparticle manipulation. Despite the remarkable capability and success, optical tweezers have notable limitations, such as complex and bulky instrumentation, high equipment costs, and potential damage to cells. To overcome the limitations of optical tweezers and other particle manipulation methods, we have developed a series of acoustic-based, on-chip devices (Figure to the left) called acoustic tweezers that can manipulate cells and other bioparticles using sound waves. Cells viability and proliferation assays were conducted to confirm the non-invasiveness of our technique. The simple structure/setup of these acoustic tweezers can be integrated with a small radio-frequency power supply and basic electronics to function as a fully integrated, portable, and inexpensive cell-manipulation system. Along with my colleagues, I have demonstrated that our acoustic tweezers can achieve the following functions: 1) single cell/organism manipulation; 2) tunable cell patterning; 3) multichannel cell sorting; and 4) high-efficiency cell separation.
Top 5 Awards and honors (name of award, date received):
5 Recent Papers:
*See Instructions Below Before Filling In This Field*